Detailed view for TcCLB.508075.14

Basic information

TDR Targets ID: 38242
Trypanosoma cruzi, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.089 | Length (AA): 413 | MW (Da): 45656 | Paralog Number: 3

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00149   Calcineurin-like phosphoesterase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016787   hydrolase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
16 318 1v73 (A) 54 336 20.00 0 1 0.85 -0.6
33 264 1v73 (A) 72 290 28.00 0 1 0.96 -0.94
22 314 2z72 (A) 62 337 22.00 0 1 0.905243 -0.05
33 129 2hy1 (A) 15 116 26.00 0 0.73 0.508667 -0.24
281 318 4uql (Q) 271 314 39.00 0.72 0.32 0.14971 2.09

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Smircich P
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile epimastigote. Smircich P
Show/Hide expression data references
  • Smircich P Ribosome profiling reveals translation control as a key mechanism generating differential gene expression in Trypanosoma cruzi.

Orthologs

Ortholog group members (OG5_130954)

Species Accession Gene Product
Arabidopsis thaliana AT1G07010   shewenella-like protein phosphatase 1
Arabidopsis thaliana AT1G18480   shewenella-like protein phosphatase 2
Cryptosporidium hominis Chro.80040   hypothetical protein
Cryptosporidium parvum cgd8_290   serine-threonine protein phosphatase
Leishmania braziliensis LbrM.29.0380   hypothetical protein, conserved
Leishmania braziliensis LbrM.22.1390   Serine-threonin protein phosphatase, putative
Leishmania donovani LdBPK_290450.1   hypothetical protein, conserved
Leishmania donovani LdBPK_221340.1   Serine-threonin protein phosphatase, putative
Leishmania infantum LinJ.29.0450   hypothetical protein, conserved
Leishmania infantum LinJ.22.1340   Serine-threonin protein phosphatase, putative
Leishmania major LmjF.29.0440   hypothetical protein, conserved
Leishmania major LmjF.22.1490   Serine-threonin protein phosphatase, putative
Leishmania mexicana LmxM.22.1490   Serine-threonin protein phosphatase, putative
Leishmania mexicana LmxM.08_29.0440   hypothetical protein, conserved
Neospora caninum NCLIV_009030   Metallophosphoesterase, related
Oryza sativa 4348542   Os10g0394100
Oryza sativa 4350234   Os11g0261900
Plasmodium berghei PBANKA_1332400   shewanella-like protein phosphatase 1
Plasmodium falciparum PF3D7_1469200   shewanella-like protein phosphatase 1, putative
Plasmodium knowlesi PKNH_1211800   shewanella-like protein phosphatase 1, putative
Plasmodium vivax PVX_117005   shewanella-like protein phosphatase 1, putative
Plasmodium yoelii PY03645   Ser/Thr protein phosphatase, putative
Trypanosoma brucei gambiense Tbg972.6.460   serine/threonine protein phosphatase, putative
Trypanosoma brucei Tb927.6.750   kinetoplastid-specific phospho-protein phosphatase, putative
Trypanosoma cruzi TcCLB.511825.170   serine/threonine protein phosphatase, putative
Trypanosoma cruzi TcCLB.508409.329   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508075.14   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508323.190   serine/threonine protein phosphatase, putative
Toxoplasma gondii TGME49_254770   Ser/Thr phosphatase family protein

Essentiality

TcCLB.508075.14 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.750 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.750 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.750 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.750 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_254770 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TcCLB.508075.14 (Trypanosoma cruzi), hypothetical protein, conserved
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